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Realtid i Vitro övervakning av luktreceptor aktivering av en Luktämnet i gasfasen
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1Department of Molecular Genetics and Microbiology,Duke University Medical Center, 2Department of Biotechnology and Life Science,Tokyo University of Agriculture and Technology, 3Department of Neurobiology,Duke University Medical Center, 4Department of Mechanical Systems, Engineering,Tokyo University of Agriculture and Technology, 5Institute of Global Innovation Research,Tokyo University of Agriculture and Technology, 6Duke Institute for Brain Sciences,Duke University
Chapters
- 00:04Title
- 00:42Hana3A Cells Culture
- 02:26Preparation of the Cells for Transfection
- 03:23Plasmid Transfection
- 05:01Substrate Incubation
- 06:07Odorant Stimulation and Data Analysis
- 08:07Results: Real-time Activation and Dose Dependent Responses
- 09:21Conclusion
Fysiologiskt, som luktämnen receptorer aktiveras av luktämnet molekyler inandning i gasfasen. Men, mest in vitro-system använda flytande fas luktämnet stimulering. Här presenterar vi en metod som möjliggör realtidsövervakning av in vitro-av luktämnet receptor aktiveringen vid luktämnet stimulering i gasfasen.
