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In tempo reale In Vitro monitoraggio dell'attivazione del recettore Odorant da un Odorant in fase gassosa
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1Department of Molecular Genetics and Microbiology,Duke University Medical Center, 2Department of Biotechnology and Life Science,Tokyo University of Agriculture and Technology, 3Department of Neurobiology,Duke University Medical Center, 4Department of Mechanical Systems, Engineering,Tokyo University of Agriculture and Technology, 5Institute of Global Innovation Research,Tokyo University of Agriculture and Technology, 6Duke Institute for Brain Sciences,Duke University
Chapters
- 00:04Title
- 00:42Hana3A Cells Culture
- 02:26Preparation of the Cells for Transfection
- 03:23Plasmid Transfection
- 05:01Substrate Incubation
- 06:07Odorant Stimulation and Data Analysis
- 08:07Results: Real-time Activation and Dose Dependent Responses
- 09:21Conclusion
Fisiologicamente, odorant recettori sono attivati da molecole odorant inalati in fase gassosa. Tuttavia, più sistemi in vitro utilizzano stimolazione odorant fase liquida. Qui, presentiamo un metodo che consente il monitoraggio in tempo reale in vitro dell'attivazione del recettore odorant stimolazione odorant in fase vapore.
