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Toksicitetsundersøgelse af zinkoxid nanopartikler i cellekultur og i Drosophila melanogaster
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Immunology and Infection
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JoVE Journal Immunology and Infection
Toxicity Study of Zinc Oxide Nanoparticles in Cell Culture and in Drosophila melanogaster

Toksicitetsundersøgelse af zinkoxid nanopartikler i cellekultur og i Drosophila melanogaster

DOI:
10.3791/59510-v

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06:47 min

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September 19, 2019

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, , , ,
1NUS Environmental Research Institute & Department of Anatomy,National University of Singapore, 2NUS Environmental Research Institute & School of Public Health,National University of Singapore, 3Department of Civil and Environmental Engineering,National University of Singapore, 4Department of Anatomy,National University of Singapore

Chapters

  • 00:04Title
  • 00:42Fluorescence Activated Cell Sorting (FACS) Analysis on Lived/Fixed Cells
  • 02:12Exposure of ZnO NPs to Drosophila
  • 03:18Dissection of Fly and ROS Detection
  • 04:22Measuring Fluorescence using ImageJ Software
  • 05:03Results: Toxicity Study of ZnO NPs
  • 05:34Conclusion

Summary

Automatic Translation

Automatic Translation

Vi beskriver en detaljeret protokol til evaluering af de toksikologiske profiler af zinkoxid nanopartikler (ZnO NPs) i særdeleshed, typen af celledød i humane MRC5 lunge fibroblaster og ROS dannelse i frugtflue Drosophila.

Tags

NanotoxicityCell DeathReactive Oxygen SpeciesFluorescence Activated Cell SortingDHE ProbeZinc Oxide NanoparticlesMRC5 Human Lung FibroblastsFITC Annexin VPropidium IodideFlow CytometryDrosophila MelanogasterFly Food

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