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JoVE Journal Biochemistry
Atomic absorbansen spektroskopi å måle intracellulære zinc Pools i pattedyrceller
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Atomic absorbansen spektroskopi å måle intracellulære zinc Pools i pattedyrceller

Article DOI: 10.3791/59519-v • 13:04 min • May 16th, 2019
May 16th, 2019 •
*1, *2, 3, 4, 2, 1
1Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, 2Department of Chemistry, Skidmore College, 3Candiac MR Center, Beth Israel Deaconess Medical Center, 4Facultad de Ciencias Químico Biológicas, Universidad Autónoma de Guerrero
* These authors contributed equally

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Kultivert primære eller etablerte cellelinjer er ofte brukt til å ta grunnleggende biologiske og mekanistisk spørsmål som en første tilnærming før du bruker dyremodeller. Denne protokollen beskriver hvordan man skal tilberede hele celle ekstrakter og subcellulære fraksjoner for studier av sink (Zn) og andre sporstoffer med Atomic absorbansen spektroskopi.

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Biokjemi sink Atomic absorbansen spektroskopi spredning differensiering pattedyr cellekultur subcellulære fraksjonering
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