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Biochemistry
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从鸡蛋黄中分离脂蛋白颗粒,研究细菌病原体脂肪酸并入膜磷脂
JoVE Journal
Biochemistry
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JoVE Journal
Biochemistry
Isolation of Lipoprotein Particles from Chicken Egg Yolk for the Study of Bacterial Pathogen Fatty Acid Incorporation into Membrane Phospholipids
Please note that all translations are automatically generated.
Click here for the English version.
从鸡蛋黄中分离脂蛋白颗粒,研究细菌病原体脂肪酸并入膜磷脂
DOI:
10.3791/59538-v
•
11:59 min
•
May 15, 2019
•
Phillip C. Delekta
,
Todd A. Lydic
,
Neal D. Hammer
1
Department of Microbiology and Molecular Genetics
,
Michigan State University
,
2
Department of Physiology
,
Michigan State University
Chapters
00:04
Title
01:01
Fractionation of LDL (Low-density Lipoprotein) Containing Plasma from Chicken Egg Yolk
02:04
Isolation of LDL Particles from Plasma
03:16
Incubation of
S. aureus
with LDLs for Membrane Lipid Analysis
05:18
Extraction of
S. aureus
Membrane Lipids
06:54
Analysis of
S. aureus
Lipid Profiles using High Resolution/Accurate Mass Spectrometry
08:26
Database Searching to Identify Endogenous
S. aureus
and Exogenous LDL-derived Lipids
09:54
Results: Fatty Acid Composition of
S. aureus
Phospholipids Cultured with Chicken LDLs
11:29
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该方法为研究将来自复杂宿主来源的外源脂肪酸结合到细菌膜中提供了一个框架,特别是
金黄色葡萄球菌。
为此,介绍了从鸡蛋蛋黄中浓缩脂蛋白颗粒的方案,以及利用质谱法对细菌磷脂进行随后脂肪酸分析的介绍。
Tags
Lipoprotein Particles
Chicken Egg Yolk
Bacterial Pathogen
Fatty Acid Incorporation
Membrane Phospholipids
Lipidomic Analysis
Staphylococcus Aureus
Ammonium Sulfate
Dialysis
Article
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