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Journal / Biochemistry / Lokalisering av SUMO-modifisert proteiner bruke…
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Biochemistry

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JoVE Journal Biochemistry
Lokalisering av SUMO-modifisert proteiner bruke fluorescerende Sumo-fangst proteiner
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Journal (Biochemistry)
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Studying RNA Interactors of Protein Kinase RNA-Activated during the Mammalian Cell Cycle
Journal (Biochemistry)
Studying RNA Interactors of Protein Kinase RNA-Activated during the Mammalian Cell Cycle
 
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Lokalisering av SUMO-modifisert proteiner bruke fluorescerende Sumo-fangst proteiner

Article DOI: 10.3791/59576-v • 06:23 min • April 27th, 2019
April 27th, 2019 •
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1Integrated Science Center, Biology Department, College of William & Mary

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SUMO er et essensielt og svært bevart, lite, ubiquitin-lignende endrings protein. I denne protokollen beskriver vi bruken av en stress-tolerant rekombinant SUMO-fangst protein (kmUTAG) å visualisere native, umerkede SUMO konjugater og deres lokalisering i en rekke celletyper.

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Biokjemi SUMO Smt3 SMO-1 sumoylation C. elegans PNT2 spirende GJÆR UTAG Sumo-fangst UD
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