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Journal / Biochemistry / Lokalisering af SUMO-modificerede proteiner ved…
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Biochemistry

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JoVE Journal Biochemistry
Lokalisering af SUMO-modificerede proteiner ved hjælp af fluorescerende Sumo-diffuse Rings proteiner
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Lokalisering af SUMO-modificerede proteiner ved hjælp af fluorescerende Sumo-diffuse Rings proteiner

Article DOI: 10.3791/59576-v • 06:23 min • April 27th, 2019
April 27th, 2019 •
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1Integrated Science Center, Biology Department, College of William & Mary

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Sumo er et vigtigt og meget bevaret, lille ubiquitin-lignende ændrings-protein. I denne protokol beskriver vi brugen af et stress-tolerant rekombinant SUMO-diffuse Rings protein (kmUTAG) til at visualisere indfødte, ukodede SUMO-konjugater og deres lokalisering i en række forskellige celletyper.

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Biokemi SUMO Smt3 SMO-1 sumoylation C. elegans PNT2 spirende GÆR UTAG Sumo-fældefangst ud
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