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Biochemistry

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JoVE Journal Biochemistry
Lokalisering av SUMO-modifierade proteiner med fluorescerande Sumo-fångst proteiner
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Lokalisering av SUMO-modifierade proteiner med fluorescerande Sumo-fångst proteiner

Article DOI: 10.3791/59576-v • 06:23 min • April 27th, 2019
April 27th, 2019 •
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1Integrated Science Center, Biology Department, College of William & Mary

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SUMO är ett viktigt och starkt bevarade, små ubiquitinliknande modifierande protein. I detta protokoll beskriver vi användningen av ett stress-tolerant rekombinant SUMO-svällningsprotein (kmUTAG) för att visualisera infödda, otaggade SUMO-konjugat och deras lokalisering i en mängd olika cell typer.

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Biokemi SUMO Smt3 SMO-1 sumoylation C. elegans PNT2 spirande jäst UTAG Sumo-SVÄLLNING UD
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