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Journal / Immunology and Infection / Effektiv transfeksjon av in vitro transkribert…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Effektiv transfeksjon av in vitro transkribert mRNA i dyrkede celler ved bruk av peptid-poloksamin nanopartikler
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Effektiv transfeksjon av in vitro transkribert mRNA i dyrkede celler ved bruk av peptid-poloksamin nanopartikler

Article DOI: 10.3791/64288-v • 10:16 min • August 17th, 2022
August 17th, 2022 •
*1, *1, 1, 1, 1, 1, 1, 2, 1, 3, 1
1National Engineering Research Center of Immunological Products, Department of Microbiology and Biochemical Pharmacy, Third Military Medical University, 2Department of Pediatrics, Ludwig-Maximilians University of Munich, 3Department of Pharmacy, Southwest Hospital, Third Military Medical University
* These authors contributed equally

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En selvmontert peptid-poloxamin nanopartikkel (PP-sNp) er utviklet ved hjelp av en mikrofluidisk blandingsanordning for å innkapsle og levere in vitro transkribert messenger RNA. Det beskrevne mRNA/PP-sNp kunne effektivt transfektere dyrkede celler in vitro.

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Immunologi og infeksjon utgave 186 Genlevering in vitro transkribert messenger RNA leveringssystemer poloksamin nanopartikkel
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