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Journal / Bioengineering / Standardisert og skalerbar analyse for å studere…
JoVE Journal
Bioengineering

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JoVE Journal Bioengineering
Standardisert og skalerbar analyse for å studere Perfusert 3D angiogenic spirende av iPSC-avledet endothelial Cells in vitro
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Standardisert og skalerbar analyse for å studere Perfusert 3D angiogenic spirende av iPSC-avledet endothelial Cells in vitro

Article DOI: 10.3791/59678-v • 10:47 min • November 6th, 2019
November 6th, 2019 •
1,2, 1, 3, 3, 4, 5, 2, 1
1Department of Internal Medicine (Nephrology) and the Einthoven Laboratory for Vascular and Regenerative Medicine, Leiden University Medical Center, 2Division of Systems Biomedicine and Pharmacology, Department of Analytical BioSciences and Metabolomics, Leiden University, 3Ncardia, 4Department of Anatomy and Embryology, Leiden University Medical Center, 5Mimetas

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Denne metoden beskriver kulturen i iPSC-avledet endothelial celler som 40 perfusert 3D microårene i en standardisert mikrovæskebasert plattform. Denne plattformen muliggjør studiet av gradient-drevet angiogenic spirende i 3D, inkludert anastomose og stabilisering av angiogenic spirer i en skalerbar og høy gjennomstrømming måte.

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Bioteknologi 3D cellekultur materialer in vitro Human indusert Pluripotent stamceller endothelial celler angiogenese graderinger høy gjennomstrømming screening analysen utvikling
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