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JoVE Journal
Bioengineering

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Generation and Quantitative Characterization of Functional and Polarized Biliary Epithelial Cysts
 

Generation and Quantitative Characterization of Functional and Polarized Biliary Epithelial Cysts

Article DOI: 10.3791/61404-v
May 16th, 2020

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Summary May 16th, 2020

Three-dimensional (3D) cellular systems are relevant models for investigating organogenesis. A hydrogel-based method for biliary cysts production and their characterization is proposed. This protocol unravels the barriers of 3D characterization, with a straightforward and reliable method to assess cyst formation efficiency, sizes, and to test their functionality.

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