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Generation and Quantitative Characterization of Functional and Polarized Biliary Epithelial Cysts
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Generation and Quantitative Characterization of Functional and Polarized Biliary Epithelial Cysts

Generation and Quantitative Characterization of Functional and Polarized Biliary Epithelial Cysts

DOI:
10.3791/61404-v

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09:55 min

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May 16, 2020

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, , , ,
1Physiopathogénèse et traitement des maladies du foie,Université Paris-Saclay, Inserm U1193, 2ESPCI Paris, Université PSL

Chapters

  • 00:04Introduction
  • 01:02Chamber Slide Preparation
  • 01:59Normal Rat Cholangiocyte Preparation
  • 03:05Cyst Generation
  • 04:29Cyst Imaging
  • 05:29Image Processing
  • 06:14Cyst Quantification
  • 07:20Results: Representative Cyst Formation, Viability, Functionality, and Polarity Analyses
  • 09:03Conclusion

Summary

Automatic Translation

Automatic Translation

Three-dimensional (3D) cellular systems are relevant models for investigating organogenesis. A hydrogel-based method for biliary cysts production and their characterization is proposed. This protocol unravels the barriers of 3D characterization, with a straightforward and reliable method to assess cyst formation efficiency, sizes, and to test their functionality.

Tags

Biliary Epithelial CystsHydrogelNormal Rat CholangiocytesCell CultureCyst FormationQuantitative AssessmentTubular StructuresBioengineeringTherapeutic TargetsDisease Mechanisms

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