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Immunology and Infection

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JoVE Journal Immunology and Infection
Kvantitativ fluorescens in situ-hybridisering (fisk) og immunofluorescens (IF) af specifikke genprodukter i KSHV-inficerede celler
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Kvantitativ fluorescens in situ-hybridisering (fisk) og immunofluorescens (IF) af specifikke genprodukter i KSHV-inficerede celler

Article DOI: 10.3791/59697-v • 06:21 min • August 27th, 2019
August 27th, 2019 •
1, 2
1Norfolk Academy, 2Department of Molecular Biophysics and Biochemistry, Howard Hughes Medical Institute, Yale School of Medicine

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Vi beskriver en protokol, der udnytter fluorescens in situ-hybridisering (FISH) til at visualisere flere herpes viral RNAs i lytisk inficerede humane celler, enten i suspension eller overholder. Denne protokol omfatter kvantificering af fluorescens, der producerer et nukleocytoplasmic-forhold, og kan udvides til samtidig visualisering af værts-og virale proteiner med immunofluorescens (IF).

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Immunologi og infektion RNA fisk hvis herpesvira kvantificering nukleocytoplasmic Shift suspension celler KSHV
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