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JoVE Journal
Immunology and Infection

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KSHV感染细胞中特定基因产物的定量原位荧光杂交(FISH)和免疫荧光(IF)
 
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KSHV感染细胞中特定基因产物的定量原位荧光杂交(FISH)和免疫荧光(IF)

Article DOI: 10.3791/59697-v 06:21 min August 27th, 2019
August 27th, 2019

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Summary

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我们描述了一种利用荧光原位杂交(FISH)来可视化被神经感染的人体细胞中的多种疱疹病毒RNA的协议,无论是悬浮的还是粘附的。该协议包括荧光的定量,产生核细胞质比,并可扩展用于同时可视化具有免疫荧光(IF)的宿主和病毒蛋白。

Tags

免疫学和感染,第150期,RNA FISH,IF,疱疹病毒,定量,核质转移,悬浮细胞,KSHV
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