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Immunology and Infection

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Separation of Rat Epidermis and Dermis with Thermolysin to Detect Site-Specific Inflammatory mRNA and Protein
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Separation of Rat Epidermis and Dermis with Thermolysin to Detect Site-Specific Inflammatory mRNA and Protein

Article DOI: 10.3791/59708-v • 08:45 min • September 29th, 2021
September 29th, 2021 •
*1, *1, *1, 1, 2, 1
1Department of Anatomy and Cell Biology, Oklahoma State University Center for Health Sciences, 2Institute for Shock Physics, Washington State University
* These authors contributed equally

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Summary
Automatic Translation

Presented here is a protocol for the separation of epidermis from dermis to evaluate inflammatory mediator production. Following inflammation, rat hind paw epidermis is separated from the dermis by thermolysin at 4 °C. The epidermis is then used for mRNA analysis by RT-PCR and protein evaluation by western blot and immunohistochemistry.

Tags

Epidermis Dermis Thermolysin Inflammatory MRNA Protein Separation Technique Site-specific Production Neurotrophins Skin Inflammation Enzymatic Separation Four Degrees Celsius MRNA Integrity Protein Integrity Mediators Primary Afferent Terminals Acute Inflammation Chronic Inflammation Therapeutic Interventions Wound Healing Skin Disease Chronic Wounds Burns Research Epithelial Surfaces GI Tract Cornea Optimal Separation Time Preliminary Experiment 2D Morphology Evaluation Pedal Reflex Response Anesthetized Rat Glabrous Hind Paw Injection Lambda Carrageenan PBS Solution Edema Volume Measurement
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