JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Biochemistry
/
富玛莱酸氢酶含含蛋白质的表达、纯化、结晶和酶测定
JoVE Journal
Biochemistry
This content is Free Access.
JoVE Journal
Biochemistry
Expression, Purification, Crystallization, and Enzyme Assays of Fumarylacetoacetate Hydrolase Domain-Containing Proteins
Please note that all translations are automatically generated.
Click here for the English version.
富玛莱酸氢酶含含蛋白质的表达、纯化、结晶和酶测定
DOI:
10.3791/59729-v
•
10:21 min
•
June 20, 2019
•
Alexander K. H. Weiss
2
,
Max Holzknecht
2
,
Elia Cappuccio
2
,
Ilaria Dorigatti
,
Karin Kreidl
,
Andreas Naschberger
,
Bernhard Rupp
,
Hubert Gstach
,
Pidder Jansen-Dürr
2
1
Research Institute for Biomedical Aging Research
,
University of Innsbruck Austria
,
2
Center for Molecular Biosciences Innsbruck (CMBI)
,
University of Innsbruck Austria
,
3
Division of Genetic Epidemiology
,
Medical University of Innsbruck Austria
,
4
Faculty of Chemistry, Department of Organic Chemistry
,
University of Vienna Austria
Chapters
00:04
Title
01:12
Expression of FAHD Proteins in Competent
E. coli
03:44
Lysis of Bacterial Pellets and Filtration of Debris
04:33
Purification of
His
-tagged FAHD Proteins using Ni-NTA Affinity Chromatography
05:59
Purification of FAHD Proteins via Ion Exchange Chromatography
06:35
Basic FAHD Activity Assays with Substrates Oxaloacetate and Acetylpyruvate
07:53
Results: Expression and Purification of Fumarylacetoacetate Hydrolase Domain-containing Proteins
09:19
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
用实例描述了含紫酯水酶的表达和纯化(
在
大肠杆菌、FPLC中的表达)。纯化蛋白用于结晶和抗体生产,并用于酶测定。提出了选定的光度测定法,以显示FAHD1的多功能性,如牛酸脱碳酶和丙烯酰胺水酶。
Tags
Expression
Purification
Crystallization
Enzyme Assays
Fumarylacetoacetate Hydrolase
FHD Protein
TCA Cycle
Energy Metabolism
Mitochondria
Cellular Senescence
IPTG Induction
Affinity Chromatography
FPLC
BL21-DE3 PLysS E. Coli
LB Agar
NZCYM Medium
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
热稳定,表达,纯化,和人类血清素转运蛋白结晶绑定到<em>取值</em> -citalopram
结合干湿实验室技术指导大型卷曲螺旋含有蛋白质的结晶
S100A12的表达,纯化和抗菌活性
重组蛋白表达,结晶和生物物理研究<em>芽孢杆菌</em> - 保守的核苷酸焦磷酸化酶,BcMazG
哺乳动物 Bestrophin 离子通道的表达与纯化
真核硼酸背液转运体的表达、增溶及纯化
无核酸酶的表达和纯化原氧基质 3,4-二氧酶
成熟水稻的横向切片(
米
苜蓿L.)使用移液器吸头作为固定支架的扫描电子显微镜成像的内核
PCR 突变体、克隆、表达、快速蛋白质纯化协议和色氨酸合成酶野生类型和突变形式的结晶
亲和色谱法表达和纯化人基质金属蛋白酶-3
Read Article