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Journal / Bioengineering / Rotulagem de vasos sanguíneos no cérebro Teleost…
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JoVE Journal Bioengineering
Rotulagem de vasos sanguíneos no cérebro Teleost e pituitária usando a perfusão cardíaca com um DiI-fixative
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Rotulagem de vasos sanguíneos no cérebro Teleost e pituitária usando a perfusão cardíaca com um DiI-fixative

Article DOI: 10.3791/59768-v • 05:53 min • June 13th, 2019
June 13th, 2019 •
1, 1
1Department of Basic Sciences and Aquatic Medicine, Faculty of Veterinary Medicine, Norwegian University of Life Sciences

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O artigo descreve um protocolo rápido para a rotulagem de vasos sanguíneos em um peixe teleósteo por perfusão cardíaca de DII diluído em fixador, usando Medaka (Oryzias latipes) como um modelo e focando no cérebro e tecido hipofisário.

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Bioengenharia DiI perfusão cardíaca rotulagem vasculatura vasos sanguíneos peixes cérebro hipófise
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