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Samlede CRISPR-baserede genetiske skærme i pattedyrsceller
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Samlede CRISPR-baserede genetiske skærme i pattedyrsceller

Article DOI: 10.3791/59780-v • 09:05 min • September 4th, 2019
September 4th, 2019 •
*1, *1, 1, 1, 1,2,3
1Donnelly Centre, University of Toronto, 2Department of Molecular Genetics, University of Toronto, 3Institute for Biomaterials and Biomedical Engineering, University of Toronto
* These authors contributed equally

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Crispr-Cas9-teknologien er en effektiv metode til præcist at redigere pattedyrs genomet i en hvilken som helst celletype og udgør et nyt middel til at udføre genom-dækkende genetiske skærme. Her er en detaljeret protokol, der diskuterer de nødvendige skridt til en vellykket udførelse af poolede Genome-Wide CRISPR-Cas9-skærme.

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Genetik CRISPR-Cas9 sgRNA biblioteker Toronto-knock-out Genome-dækkende CRISPR-screening puljede Drop-out skærme funktionel Genomics essentielle gener celle egnethed spredning
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