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分化肝细胞中的血管性脂细胞病的诱导和特征
JoVE Journal
Medicine
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JoVE Journal Medicine
Inducing and Characterizing Vesicular Steatosis in Differentiated HepaRG Cells
DOI:

09:15 min

July 18, 2019

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Chapters

  • 00:04Title
  • 00:32Thawing, Amplification, and Cryopreservation of HepaRG Cells
  • 02:20Differentiation of HepaRG cells (Day 0 21) and Induction of Vesicular Steatosis (Day 21 26)
  • 04:25Evaluation of Lipid Overloading and Steatosis Induction: Oil Red O Staining
  • 06:04Evaluation of Lipid Overloading and Steatosis Induction: Bodipy Staining
  • 07:02Results: Induction and Quantification of Vesicular Steatosis in HepaRG Cells
  • 08:34Conclusion

Summary

Automatic Translation

在这项研究中,我们描述了用脂肪酸盐酸钠酸化酸酸诱导分化肝细胞中肝囊性性性软体炎的详细方案,并采用脂质积累检测和定量的方法,包括相干抗斯托克斯拉曼散射 (CARS) 显微镜、细胞氟分析、油红色 O 染色和 qPCR。

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