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In vivo billeddannelse og Kvantitation af værten Angiogen respons i Zebrafish tumor Xenografts
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
In Vivo Imaging and Quantitation of the Host Angiogenic Response in Zebrafish Tumor Xenografts
DOI:

11:07 min

August 14, 2019

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Chapters

  • 00:06Title
  • 00:44Preparation of Embryos for Implantation
  • 01:31Perivitelline Injection of Mammalian Cancer Cells into 2 dpf (Day Post Fertilization) Embryos
  • 03:55Live Imaging and Quantitation of the Angiogenic Response to the Zebrafish Xenograft
  • 08:34Results: Quantitation of the Host Angiogenic Response in Zebrafish Tumor Xenografts
  • 10:37Conclusion

Summary

Automatic Translation

Formålet med denne metode er at generere en in vivo model af tumor angiogenese ved xenografting pattedyrs tumorceller i en zebra embryo, der har fluorescently-mærkede blodkar. Ved billeddannelse af xenograft og tilhørende beholdere kan der opnås en kvantitativ måling af angiogen respons.

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