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Bioengineering
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用于循环作用电位记录的多井微电极阵列上的人类 iPSC 衍生心肌细胞网络
JoVE Journal
Bioengineering
This content is Free Access.
JoVE Journal
Bioengineering
Human iPSC-Derived Cardiomyocyte Networks on Multiwell Micro-electrode Arrays for Recurrent Action Potential Recordings
Please note that all translations are automatically generated.
Click here for the English version.
用于循环作用电位记录的多井微电极阵列上的人类 iPSC 衍生心肌细胞网络
DOI:
10.3791/59906-v
•
08:53 min
•
July 15, 2019
•
Viviana Zlochiver*
1
,
Stacie L. Kroboth*
1
,
Christopher R. Beal
,
Jonathan A. Cook
,
Rosy Joshi-Mukherjee
2,3
1
Aurora Research Institute
,
Advocate Aurora Health Care
,
2
Department of Biomedical Engineering, College of Engineering and Applied Science
,
University of Wisconsin-Milwaukee
,
3
Department of Medicine-Cardiovascular, School of Medicine
,
Johns Hopkins University
Chapters
00:04
Title
00:56
Pre-plating of the Cryopreserved hiPSC-CM for Maturation
01:35
Multiwell MEA Plate Sterilization and Coating
02:57
hiPSC-CM Dissociation and Plating on Multiwell MEA Plate
04:09
hiPSC-CM Electroporation and Signal Acquisition
05:06
Data Segmentation and Analysis
06:40
Results: Action Potential Recordings of hiPSC-CMs
08:21
Conclusion
Summary
Automatic Translation
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Automatic Translation
本文包含一套用于开发人类诱导多能干细胞衍生心肌细胞(hiPSC-CM)网络的协议,这些网络在多孔MEA板上培养,可逆电化细胞膜进行作用电势测量。高通量记录在几天内从同一单元站点重复获取。
Tags
Human IPSC-derived Cardiomyocytes
Multiwell Micro-electrode Arrays
Action Potential Recordings
Cell Plating
Electrophysiology
MATLAB Workflow
Signal-to-noise Ratio
Fibronectin Coating
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