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Creating a Structurally Realistic Finite Element Geometric Model of a Cardiomyocyte to Study the Role of Cellular Architecture in Cardiomyocyte Systems Biology
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1Cell Structure and Mechanobiology Group,University of Melbourne, 2Systems Biology Laboratory, Melbourne School of Engineering,University of Melbourne, 3Department of Biomedical Engineering,University of Melbourne, 4School of Mathematics and Statistics, Faculty of Science,University of Melbourne, 5Department of Engineering Science,University of Auckland, 6Advanced Microscopy Facility, Bio21 Molecular Science and Biotechnology Institute,University of Melbourne, 7ARC Centre of Excellence in Convergent Bio-Nano Science and Technology,University of Melbourne, 8School of Medicine, Faculty of Medicine, Dentistry and Health Sciences,University of Melbourne, 9Living Systems Institute,University of Exeter
Chapters
- 00:04Title
- 01:19Segment Myofibrils and Mitochondria Regions from the EM 3D Image Dataset
- 03:05Create a Finite Element Mesh from the Segmented Components
- 04:36Mathematically Map the Spatially Varying Density of Ion-channels of Interest onto the Finite Element Mesh
- 06:51Results: A Structurally Realistic Finite Element Geometric Model of a Cardiomyocyte
- 08:13Conclusion
This protocol outlines a novel method to create a spatially detailed finite element model of the intracellular architecture of cardiomyocytes from electron microscopy and confocal microscopy images. The power of this spatially detailed model is demonstrated using case studies in calcium signaling and bioenergetics.
