Fabricating Multi-Component Lipid Nanotube Networks Using the Gliding Kinesin Motility Assay

Journal

Bioengineering

JoVE Journal
Bioengineering

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JoVE Journal Bioengineering

Fabricating Multi-Component Lipid Nanotube Networks Using the Gliding Kinesin Motility Assay

DOI:

10.3791/60899-v

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05:16 min

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July 26, 2021

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Zachary I. Imam, George D. Bachand

¹Center for Integrated Nanotechnologies,Sandia National Laboratories

Chapters

00:04Introduction
00:32Gliding Motility Assay (GMA)
01:55Lipid Nanotube (LNT) Network Imaging
02:31LNT Network Characterization
03:41Results: Representative LNTs Fabricated from Different GUV Lipid Formulations
04:36Conclusion

Summary

Automatic Translation

This protocol describes a process for fabricating lipid nanotube networks using gliding kinesin motility in conjunction with giant unilamellar lipid vesicles.

Fabricating Multi-Component Lipid Nanotube Networks Using the Gliding Kinesin Motility Assay

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