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Visualisere Surface T-Cell Receptor Dynamics Fire-Dimensionelt Ved hjælp af gitter light-sheet mikroskopi
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Visualizing Surface T-Cell Receptor Dynamics Four-Dimensionally Using Lattice Light-Sheet Microscopy
DOI:

09:24 min

January 30, 2020

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Chapters

  • 00:04Introduction
  • 01:22Prepare Cells
  • 03:40Conducting LLSM Daily Alignment
  • 05:13Setting up Cells with LLSM
  • 07:03Results: Four-dimensional Visualization of Surface T-cell Receptor Dynamics
  • 08:10Conclusion

Summary

Automatic Translation

Målet med denne protokol er at vise, hvordan man bruger Lattice Light-Sheet Mikroskopi til firedimensionelt visualisere overflade receptor dynamik i levende celler. Her vises T-cellereceptorer på CD4+ primære T-celler.

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