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Chapters
- 00:04Introduction
- 00:57Isolation of Mouse Adipocyte Precursor Cells
- 06:33Adipocyte Differentiation and Induction of Insulin Resistance
- 08:14Results: Differentiation of Adipocyte Precursor Cells from Subcutaneous Fat into Mature Adipocytes and Insulin Resistance Induced by TNF‐α in Primary Adipocytes
- 09:16Conclusion
This protocol describes the isolation of mouse preadipocytes from subcutaneous fat, their differentiation into mature adipocytes, and the induction of insulin resistance. Insulin action is evaluated by the phosphorylation/activation of members of the insulin signaling pathway through western blot. This method allows direct determination of insulin resistance/sensitivity in primary adipocytes.
