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Demonstration of Heterologous Complexes formed by Golgi-Resident Type III Membrane Proteins using Split Luciferase Complementation Assay
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Demonstration of Heterologous Complexes formed by Golgi-Resident Type III Membrane Proteins using Split Luciferase Complementation Assay

Article DOI: 10.3791/61669-v • 05:28 min • September 10th, 2020
September 10th, 2020 •
1, 1, 1
1Laboratory of Biochemistry, Faculty of Biotechnology, University of Wroclaw

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The protocol presented here is intended to demonstrate the occurrence of heterologous interactions between Golgi-resident type III membrane proteins with cytoplasmically exposed N- and/or C-termini in live mammalian cells using the most recent variant of the split luciferase complementation assay.

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Heterologous Complexes Golgi-resident Type III Membrane Proteins Split Luciferase Complementation Assay Multi Transmembrane Proteins Endoplasmic Reticulum Golgi Complex Living Cells Low Expression Levels Special Sensitivity High Over Expression Levels Cellular Health Protein Subcellular Localization Adherent HEK293 T-cell Culture Trypsinization Complete Growth Medium 96-well Plate Transfection Expression Plasmids Serum-free Medium Lipid-based Transfection Reagent Lipid DNA Mixture
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