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Anvendelse af frosset væv i Comet-analysen til vurdering af DNA-skader
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Anvendelse af frosset væv i Comet-analysen til vurdering af DNA-skader

Article DOI: 10.3791/59955-v • 11:30 min • March 24th, 2020
March 24th, 2020 •
*1, 1, 1, 2
1Toxicology Program, ILS, Inc., 2Division of the National Toxicology Program, National Institute of Environmental Health Sciences
* These authors contributed equally

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Denne protokol beskriver flere procedurer for fremstilling af frosne vævsprøver af høj kvalitet på tidspunktet for obduktion til brug i kometanalysen til vurdering af DNA-skader: 1) hakket væv, 2) skrabede epitelceller fra mave-tarmkanalen og 3) ternet væv prøver, der kræver homogenisering ved hjælp af en vævshakkeanordning.

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Genetik enkeltcellegelelektroforese alkalisk kometanalyse kromosomale skader DNA-strengbrud genotoksicitet in vivo OECD
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