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Journal / Genetics / Identificering af mutationer ved høj opløsning…
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Identificering af mutationer ved høj opløsning smelter i en TILLING population af ris
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Identificering af mutationer ved høj opløsning smelter i en TILLING population af ris

Article DOI: 10.3791/59960-v • 06:10 min • September 2nd, 2019
September 2nd, 2019 •
1, 1, 1, 2, 3, 1, 4
1National Key Laboratory of Rice Biology, Zhejiang Provincial Key Laboratory of Crop Genetic Resources, Institute of Crop Science, Zhejiang University, 2Jiaxing Academy of Agricultural Sciences, 3Institute of Nuclear Agricultural Sciences, Zhejiang University, 4The New Countryside Development Institute at Zhejiang University

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I denne artikel præsenterer vi den protokol, der er beskrevet som høj opløsning smelte analyse (HRM)-baseret Target induceret lokale læsioner i genomer (TILLING). Denne metode udnytter fluorescens ændringer under smeltning af DNA-duplex og er egnet til høj gennemløbs screening af både indsættelse/sletning (Indel) og enkelt base substition (SBS).

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Genetik høj opløsning smelte analyse HRM TILLING mutation screening Indel og SBS gammastråler ris
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