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JoVE Journal Genetics
Brug af næste generations sekventering til at identificere mutationer forbundet med reparation af et CAS9-induceret dobbeltstrengsbrud nær CD4-promotoren
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Brug af næste generations sekventering til at identificere mutationer forbundet med reparation af et CAS9-induceret dobbeltstrengsbrud nær CD4-promotoren

Article DOI: 10.3791/62583-v • 06:59 min • March 31st, 2022
March 31st, 2022 •
*1, *2, 1, 1, 2,3
1Division of Biology, Kansas State University, 2Kansas State Veterinary Diagnostic Laboratory, Kansas State University, 3Department of Diagnostic Medicine/Pathobiology, Kansas State University
* These authors contributed equally

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Præsenteret her er sgRNA / CAS9 endonuklease og næste generations sekventeringsprotokol, der kan bruges til at identificere mutationerne forbundet med dobbeltstrengbrudsreparation nær CD4-promotoren.

Tags

Genetik Udgave 181 DNA-skaderespons DSB DSB-reparation CAS9-induceret DSB Næste generations sekventering genomik
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