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Journal / Genetics / Introduktion af punktmutationer i humane…
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Genetics

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JoVE Journal Genetics
Introduktion af punktmutationer i humane pluripotente stamceller ved hjælp af problemfri genomredigering
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Introduktion af punktmutationer i humane pluripotente stamceller ved hjælp af problemfri genomredigering

Article DOI: 10.3791/61152-v • 09:03 min • May 10th, 2020
May 10th, 2020 •
1, 1, 1
1MRC Centre for Regenerative Medicine, University of Edinburgh

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Her beskriver vi en detaljeret metode til problemfri genredigering i humane pluripotente stamceller ved hjælp af et piggyBac-baseret donorplasmid og Cas9 nickasemutanten. To punktmutationer blev indført i exon 8 af hepatocytkernefaktor 4 alfa (HNF4α) locus i humane embryonale stamceller (hESC'er).

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Genetik udgave 159 genomredigering CRISPR sømløse pluripotente stamceller piggyBac knock-in
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