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En enkel tilgang til at udføre TEER målinger ved hjælp af en selv lavet volt-amperemeter med programmerbar Udgangsfrekvens
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JoVE Journal Biology
A Simple Approach to Perform TEER Measurements Using a Self-Made Volt-Amperemeter with Programmable Output Frequency

En enkel tilgang til at udføre TEER målinger ved hjælp af en selv lavet volt-amperemeter med programmerbar Udgangsfrekvens

DOI:
10.3791/60087-v

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07:43 min

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October 05, 2019

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, , , , , , ,
1Interfaculty Institute of Biochemistry,University of Tübingen, 2Laboratory of Clinical Regenerative Medicine, Department of Neurosurgery, Faculty of Medicine,University of Tsukuba, 3Department of Pediatrics, Medical Faculty Mannheim,Heidelberg University

Chapters

  • 00:00Title
  • 01:59Protocol
  • 05:54Results
  • 07:08Conclusion

Summary

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Her demonstrerer vi, hvordan man opsætter en billig volt-amperemeter med programmerbar udgangsfrekvens, der kan bruges med kommercielt tilgængelige Chopstick-elektroer til transepithelial/Endothelial elektriske modstands målinger.

Tags

TEER MeasurementTransepithelial Electrical ImpedanceVolt-ammeterProgrammable Output FrequencyCell CultureBlood-cerebrospinal Fluid BarrierIn Vitro ModelChopstick ElectrodeEpithelial Volt-ammeterUSB ChargerMicrocontrollerSquare Wave CurrentMultimetersRJ14 ConnectorLow-budget

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