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Bioengineering
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トリマーエピトーププレゼンテーションを必要とするワクチンに対する
大腸菌
の自己組織化タンパク質ナノ粒子の産生
JoVE Journal
Bioengineering
This content is Free Access.
JoVE Journal
Bioengineering
Production of
E. coli
-expressed Self-Assembling Protein Nanoparticles for Vaccines Requiring Trimeric Epitope Presentation
Please note that all translations are automatically generated.
Click here for the English version.
トリマーエピトーププレゼンテーションを必要とするワクチンに対する
大腸菌
の自己組織化タンパク質ナノ粒子の産生
DOI:
10.3791/60103-v
•
00:10 min
•
August 21, 2019
•
Christopher P. Karch
2
,
Peter Burkhard
,
Gary R. Matyas
,
Zoltan Beck
2
1
U.S. Military HIV Research Program
,
Walter Reed Army Institute of Research
,
2
Henry M. Jackson Foundation for the Advancement of Military Medicine
,
3
Alpha-O Peptides AG
Chapters
00:04
Title
00:43
Lysis of E. Coli BL21(DE3) Containing Six-Helix Bundle Self-assembling Protein Nanoparticles
01:24
Protein Purification
05:14
Purity Assessment and Protein Identification
06:28
SHB-SAPN Refolding
07:46
Dynamic Light Scattering (DLS)
08:30
Results: Representative SHB-SAPN Characterization
10:07
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
ここでは、ワクチン開発に使用する自己組織化タンパク質ナノ粒子(SAPN)の精製、再折りたたみ、および特性評価について詳しく説明します。
Tags
E. Coli
Self-assembling Protein Nanoparticles
SAPN
Vaccines
Trimeric Epitope Presentation
Protein Purification
Protein Refolding
Protein Characterization
Six-helix Bundle
FPLC
Affinity Chromatography
HisTrap HP Column
Protein Expression
Large-scale Production
Clinical Trials
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