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Journal / Developmental Biology / Isolering av spesifikke Nevron populasjoner fra…
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Developmental Biology

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JoVE Journal Developmental Biology
Isolering av spesifikke Nevron populasjoner fra roundworm Caenorhabditis elegans
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Isolering av spesifikke Nevron populasjoner fra roundworm Caenorhabditis elegans

Article DOI: 10.3791/60145-v • 09:42 min • August 6th, 2019
August 6th, 2019 •
1, 1, 1,2,3,4
1Department of Biochemistry, University of Nebraska, 2Nebraska Redox Biology Center, University of Nebraska, 3Nebraska Center for Integrated Biomolecular Communication, University of Nebraska, 4Fred & Pamela Buffett Cancer Center, University of Nebraska Medical Center

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Her presenterer vi en protokoll for enkel isolering av spesifikke grupper av levende neuronal celler som uttrykker grønt fluorescerende protein fra transgene Caenorhabditis elegans linjer. Denne metoden gjør det mulig for en rekke ex vivo studier fokusert på spesifikke neurons og har kapasitet til å isolere celler for ytterligere kortsiktige dyrking.

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Utviklingsbiologi C. elegans celle isolering neurons aldring neuronal markører grønt fluorescerende protein
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