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Journal
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Biochemistry
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细胞和组织中辅酶A的定量
JoVE Journal
Biochemistry
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JoVE Journal
Biochemistry
Quantification of Coenzyme A in Cells and Tissues
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细胞和组织中辅酶A的定量
DOI:
10.3791/60182-v
•
08:51 min
•
September 27, 2019
•
Matthew W. Frank
,
Chitra Subramanian
,
Charles O. Rock
,
Suzanne Jackowski
1
Department of Infectious Diseases
,
St. Jude Children’s Research Hospital
Chapters
00:05
Title
01:30
Extraction and Derivatization of CoA (Coenzyme A) in Cultured Cells
03:32
Extraction & Derivatization of CoA in Tissues
05:31
Sample Clean-up with Solid Phase Extraction Column
06:47
Results: HPLC Profiles and CoA-bimane Standard Curves
07:45
Conclusion
Summary
Automatic Translation
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Automatic Translation
该方法描述了来自培养细胞和动物组织的样品制备,在样品中提取和衍生辅酶A,然后高压液相色谱用于纯化和定量衍生辅酶A吸光或荧光检测。
Tags
Coenzyme A
CoA
Cellular Metabolism
Neurodegeneration
Brain Iron Accumulation
CoA Biosynthetic Pathway
Sample Preparation
Solid-phase Extraction
Cell Culture
Potassium Hydroxide
Trizma-hydrochloride
MBBr
Thiol
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