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Obtention af gigantiske Unilamellar hybrid vesikler ved elektro dannelse og måling af deres mekaniske egenskaber ved mikropipette aspiration
JoVE Journal
Chemistry
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JoVE Journal Chemistry
Obtention of Giant Unilamellar Hybrid Vesicles by Electroformation and Measurement of their Mechanical Properties by Micropipette Aspiration
DOI:

09:29 min

January 19, 2020

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Chapters

  • 00:04Title
  • 01:07Glass Micropipette Tip Coating
  • 01:55Electroformation Chamber Preparation
  • 03:06Giant Unilamellar Vesicle (GUV) Electroformation
  • 04:12Micromanipulation Setup
  • 06:30Micropipette Aspiration Experiment
  • 07:57Results: Representative Stress-Strain Data for Liposomes, Polymersome, and Hybrid Polymer/Lipid Vesicles
  • 08:44Conclusion

Summary

Automatic Translation

Målet med protokollen er at pålideligt måle membran mekaniske egenskaber af gigantiske vesikler ved mikropipette aspiration.

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