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Immunology and Infection
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细菌细胞的三维成像,实现精确的细胞表现和精确的蛋白质定位
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Three-dimensional Imaging of Bacterial Cells for Accurate Cellular Representations and Precise Protein Localization
Please note that all translations are automatically generated.
Click here for the English version.
细菌细胞的三维成像,实现精确的细胞表现和精确的蛋白质定位
DOI:
10.3791/60350-v
•
06:33 min
•
October 29, 2019
•
Benjamin P. Bratton
,
Brody Barton
,
Randy M. Morgenstein
1
Department of Microbiology and Molecular Genetics
,
Oklahoma State University
,
2
Department of Molecular Biology and Lewis-Sigler Institute of Integrative Genomics
,
Princeton University
Chapters
00:04
Title
00:22
Slide Preparation
01:41
Imaging
03:05
Cell Reconstruction
05:10
Results: Representative Assessment of Protein Localization to Specific Cellular Geometries
06:00
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议解释了如何准备和安装细菌样本进行活的三维成像,以及如何从这些图像中重建
大肠杆菌
的三维形状。
Tags
3D Imaging
Bacterial Cells
Cellular Representation
Protein Localization
Microscopy
Agarose Pads
E. Coli
Z-stack
Fluorescence Imaging
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