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Developmental Biology
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斑马鱼胚胎全山原位杂交和管状测定在iPSC-ECs中研究恩多林在血管发育中的作用
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Developmental Biology
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JoVE Journal
Developmental Biology
Whole-Mount In Situ Hybridization in Zebrafish Embryos and Tube Formation Assay in iPSC-ECs to Study the Role of Endoglin in Vascular Development
Please note that all translations are automatically generated.
Click here for the English version.
斑马鱼胚胎全山原位杂交和管状测定在iPSC-ECs中研究恩多林在血管发育中的作用
DOI:
10.3791/60498-v
•
08:27 min
•
May 28, 2020
•
Yong Wang*
1
,
Ding Zhang*
2
,
Fang Zhou
,
Meijun Zhou
,
Qiujie Li
,
Jingyu Chen
,
Jun Yang
2
1
Department of Physiology and Department of Cardiology of the Second Affiliated Hospital
,
Zhejiang University School of Medicine
,
2
Department of Cell Biology, State Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences
,
Chinese Academy of Medical Sciences and Peking Union Medical College
,
3
Wuxi Lung Transplant Center
,
Wuxi People’s Hospital affiliated to Nanjing Medical University
Chapters
00:04
Introduction
01:00
Zebrafish Embryo Collection and Fixation
02:55
Amplification and Microscopy
04:27
Tube Formation Assay
06:40
Results: Defective Tube Formation After Endoglin Knockdown
07:43
Conclusion
Summary
Automatic Translation
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Automatic Translation
本文介绍的是一种在斑马鱼和管状细胞中对斑马鱼进行全位RNA杂交分析的协议,在患者诱导的多能干细胞衍生内皮细胞中进行管状分析,以研究内索林在血管形成中的作用。
Tags
Whole-mount In Situ Hybridization
WISH
Zebrafish Embryos
Tube Formation Assay
IPSC-ECs
Endoglin
Vascular Development
Microinjection
Morpholinos
MRNA
Proteinase K
Target Probes
Paraformaldehyde
SSCT
Amp 1
Amp 2
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