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Imaging Dpp Release from a Drosophila Wing Disc
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Developmental Biology
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JoVE Journal Developmental Biology
Imaging Dpp Release from a Drosophila Wing Disc

Imaging Dpp Release from a Drosophila Wing Disc

DOI:
10.3791/60528-v

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06:12 min

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October 30, 2019

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1Department of Pediatrics,University of Colorado School of Medicine

Chapters

  • 00:04Title
  • 00:42Generation of Larvae for Dissection
  • 01:51Dissection Solution and Slide Preparation
  • 02:44Dissection and Mounting for Imaging
  • 04:20Results: Dpp-GFP Release in the Wing Disc
  • 05:27Conclusion

Summary

Automatic Translation

Automatic Translation

The timing of exposure to ligands may impact their developmental consequences. Here we show how to image release of a Drosophila bone morphogenetic protein (BMP) called Dpp from cells of the wing disc.

Tags

Keywords: Dpp ReleaseDrosophila Wing DiscBMP Signaling PathwayDPP-GFPFluorescence ImagingWing Disc DissectionModified HL3 MediaThird Instar Larvae

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