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成像 Dpp 释放从果蝇翼光盘

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Developmental Biology

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JoVE Journal Developmental Biology

Imaging Dpp Release from a Drosophila Wing Disc

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成像 Dpp 释放从果蝇翼光盘

DOI:

10.3791/60528-v

•

06:12 min

•

October 30, 2019

•

Laura Faith George, Emily Anne Bates

¹Department of Pediatrics,University of Colorado School of Medicine

Chapters

00:04Title
00:42Generation of Larvae for Dissection
01:51Dissection Solution and Slide Preparation
02:44Dissection and Mounting for Imaging
04:20Results: Dpp-GFP Release in the Wing Disc
05:27Conclusion

Summary

Automatic Translation

接触配体的时机可能会影响其发育后果。在这里，我们展示如何图像释放的果蝇骨形态遗传蛋白（BMP），称为Dpp从翅膀盘的细胞。

Tags

Dpp Release Drosophila Wing Disc BMP Signaling Pathway DPP-GFP Fluorescence Imaging Wing Disc Dissection Modified HL3 Media Third Instar Larvae

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