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Journal / Biochemistry / Ring Tipi E3 Ubiquitin Ligases In Vitro ve Planta …
JoVE Journal
Biochemistry

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JoVE Journal Biochemistry
Ring Tipi E3 Ubiquitin Ligases In Vitro ve Planta Fonksiyonel Karakterizasyonu
Quantifying the Binding Interactions Between Cu(II) and Peptide Residues in the Presence and Absence of Chromophores
Journal (Biochemistry)
Quantifying the Binding Interactions Between Cu(II) and Peptide Residues in the Presence and Absence of Chromophores
Studying RNA Interactors of Protein Kinase RNA-Activated during the Mammalian Cell Cycle
Journal (Biochemistry)
Studying RNA Interactors of Protein Kinase RNA-Activated during the Mammalian Cell Cycle
 
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Ring Tipi E3 Ubiquitin Ligases In Vitro ve Planta Fonksiyonel Karakterizasyonu

Article DOI: 10.3791/60533-v • 10:27 min • December 5th, 2019
December 5th, 2019 •
1, 2, 2, 2, 2, 1, 2
1Department of Entomology, Plant Pathology and Nematology, University of Idaho, 2Department of Plant Sciences, University of Idaho

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Bu makalenin amacı, RING tipi E3 ubikitin ligaseskapsamlı biyokimyasal ve fonksiyonel çalışmalar için bir anahat sunmaktır. Ayrıntılı protokollere sahip bu çok aşamalı boru hattı, test edilen proteinin enzimatik aktivitesini doğrular ve aktivitenin işlevine nasıl bağdaştırılabildiğini gösterir.

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Biyokimya Sayı 154 ubiquitinasyon RING tipi E3 ubiquitin ligaz RHA1B efektörü Globodera pallida Nicotiana benthamiana Agrobacterium-aracılı protein geçici ekspresyonu site yönelimli mutagenez
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