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Journal / Biochemistry / RING-E3型无二体气体在Vitro和Planta中的功能特性…
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Biochemistry

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JoVE Journal Biochemistry
RING-E3型无二体气体在Vitro和Planta中的功能特性
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RING-E3型无二体气体在Vitro和Planta中的功能特性

Article DOI: 10.3791/60533-v • 10:27 min • December 5th, 2019
December 5th, 2019 •
1, 2, 2, 2, 2, 1, 2
1Department of Entomology, Plant Pathology and Nematology, University of Idaho, 2Department of Plant Sciences, University of Idaho

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本手稿的目的是为 RING 型 E3 泛性白化酶的综合生化和功能研究提供大纲。此多步骤管道，具有详细的协议，验证被测试蛋白质的酶活性，并演示如何将活动与功能联系起来。

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生物化学， 问题 154， 普及， RING型 E3 泛性基质， RHA1B 效应器，格洛博代拉帕利达，尼科蒂亚娜本塔米亚纳，农业细菌介导蛋白瞬态表达， 位点定向诱变
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