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Isolation of Adult Human Dermal Fibroblasts from Abdominal Skin and Generation of Induced Pluripotent Stem Cells Using a Non-Integrating Method
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Bioengineering
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JoVE Journal Bioengineering
Isolation of Adult Human Dermal Fibroblasts from Abdominal Skin and Generation of Induced Pluripotent Stem Cells Using a Non-Integrating Method

Isolation of Adult Human Dermal Fibroblasts from Abdominal Skin and Generation of Induced Pluripotent Stem Cells Using a Non-Integrating Method

DOI:
10.3791/60629-v

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10:52 min

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January 19, 2020

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, , , , , , ,
1Department of Public Health,University of Naples Federico II

Chapters

  • 00:04Title
  • 01:31Isolation of Human Dermal Fibroblasts
  • 02:30Expansion of Human Skin Fibroblasts
  • 04:40Reprogramming of Dermal Fibroblasts to iPSCs (Induced Pluripotent Stem Cells)
  • 08:34Results: Skin Fibroblast
  • 09:52Conclusion

Summary

Automatic Translation

Automatic Translation

Cell reprogramming requires the introduction of key genes, which regulate and maintain the pluripotent cell state. The protocol described enables the formation of induced pluripotent stem cells (iPSCs) colonies from human dermal fibroblasts without viral/integrating methods but using non-modified RNAs (NM-RNAs) combined with immune evasion factors reducing cellular defense mechanisms.

Tags

Keywords: Dermal FibroblastsInduced Pluripotent Stem CellsNon-integrating MethodSkin Punch BiopsyRegenerative MedicineGenetic DisordersCancerCell IsolationCell ReprogrammingXeno-freeAutologous IPS CellsTrypsin-EDTATSS

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