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Journal
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Immunology and Infection
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使用多盐皮下聚乙烯醇海绵植入和外向尾皮肤伤口模型进行急性伤口愈合评估。
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
Assessment of Acute Wound Healing using the Dorsal Subcutaneous Polyvinyl Alcohol Sponge Implantation and Excisional Tail Skin Wound Models.
Please note that all translations are automatically generated.
Click here for the English version.
使用多盐皮下聚乙烯醇海绵植入和外向尾皮肤伤口模型进行急性伤口愈合评估。
DOI:
10.3791/60653-v
•
09:06 min
•
March 25, 2020
•
Meredith J. Crane
,
William L. Henry Jr
,
Holly L. Tran
,
Jorge E. Albina
,
Amanda M. Jamieson
1
Department of Molecular Microbiology & Immunology
,
Brown University
,
2
Division of Surgical Research, Department of Surgery
,
Rhode Island Hospital and The Warren Alpert School of Medicine of Brown University
Chapters
00:05
Introduction
00:53
Subcutaneous Polyvinyl Alcohol (PVA) Sponge Implantation
03:09
PVA Sponge Fluid Isolation
03:58
PVA Sponge Cell Isolation
05:33
Tail Skin Excision
06:57
Results: Representative Assessment of Acute Wound Healing
08:40
Conclusion
Summary
Automatic Translation
English (Original)
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这里描述了两个鼠伤愈合模型,一个旨在评估细胞和细胞因子伤口愈合反应,另一个用于量化伤口愈合率。这些方法可用于复杂的疾病模型,如糖尿病,以确定伤口愈合不良的各个方面的机制。
Tags
Wound Healing
PVA Sponge Implantation
Excisional Tail Skin Wound
Cellular And Cytokine Kinetics
Wound Closure
Comorbid Conditions
Diabetes
Pneumonia
C57-Black/6 Mouse
Subcutaneous Pocket
PVA Sponge Implantation Procedure
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