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Immunology and Infection
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Kvantificering af staphyloccus aureus cytotoksicitet mod humane Polymorphonukleare leukocytter
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Quantifying the Cytotoxicity of Staphylococcus aureus Against Human Polymorphonuclear Leukocytes

Kvantificering af staphyloccus aureus cytotoksicitet mod humane Polymorphonukleare leukocytter

DOI:
10.3791/60681-v

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12:27 min

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January 03, 2020

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, , , , , , ,
1Department of Microbiology and Immunology,Montana State University, 2University Communications,Montana State University

Chapters

  • 00:00Title
  • 00:44Polymorphonuclear Leukocyte Purification
  • 04:34Cytotoxicity Assay of S. aureus Extracellular Proteins Against Human Polymorphonuclear Leukocytes
  • 06:16S. aureus Cytotoxicity Against Human Polymorhonuclear Leukocytes Following Phagocytosis
  • 08:40Results
  • 11:02Conclusion

Summary

Automatic Translation

Automatic Translation

Denne protokol beskriver en metode til rensning af polymorphonukleare leukocytter fra hele humant blod og to særskilte analyser, der kvantificerer cytotoksicitet af Staphylococcus aureus mod disse vigtige medfødte immunceller.

Tags

Staphylococcus AureusCytotoxicityPolymorphonuclear LeukocytesPMNsNeutrophilsBi-component LeukotoxinsMembrane IntegrityInnate Immune CellsCentrifugationDensity GradientsHeparinized Whole BloodDextranFicollCell IsolationCell Separation

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