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Journal
/
Immunology and Infection
/
量化
金黄色葡萄球
菌对人多态核白细胞的细胞毒性
JoVE Journal
Immunology and Infection
Author Produced
This content is Free Access.
JoVE Journal
Immunology and Infection
Quantifying the Cytotoxicity of
Staphylococcus aureus
Against Human Polymorphonuclear Leukocytes
Please note that all translations are automatically generated.
Click here for the English version.
量化
金黄色葡萄球
菌对人多态核白细胞的细胞毒性
DOI:
10.3791/60681-v
•
12:27 min
•
January 03, 2020
•
Jennifer G. Dankoff
,
Kyler B. Pallister
,
Fermin E. Guerra
,
Alexander J. Parks
,
Kelly Gorham
,
Saul Mastandrea
,
Jovanka M. Voyich
,
Tyler K. Nygaard
1
Department of Microbiology and Immunology
,
Montana State University
,
2
University Communications
,
Montana State University
Chapters
00:00
Title
00:44
Polymorphonuclear Leukocyte Purification
04:34
Cytotoxicity Assay of
S. aureus
Extracellular Proteins Against Human Polymorphonuclear Leukocytes
06:16
S. aureus
Cytotoxicity Against Human Polymorhonuclear Leukocytes Following Phagocytosis
08:40
Results
11:02
Conclusion
Summary
Automatic Translation
English (Original)
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français (French)
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Automatic Translation
该协议描述了从全人类血液中纯化多态核白细胞的方法,以及两种独特的测定方法,用于量化
金黄色葡萄球菌
对这些重要的先天免疫细胞的细胞毒性。
Tags
Staphylococcus Aureus
Cytotoxicity
Polymorphonuclear Leukocytes
PMNs
Neutrophils
Bi-component Leukotoxins
Membrane Integrity
Innate Immune Cells
Centrifugation
Density Gradients
Heparinized Whole Blood
Dextran
Ficoll
Cell Isolation
Cell Separation
Article
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