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JoVE Journal
Biochemistry

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Fully Processed Recombinant KRAS4b
 

Fully Processed Recombinant KRAS4b: Isolating and Characterizing the Farnesylated and Methylated Protein

Article DOI: 10.3791/60703
January 16th, 2020

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Summary January 16th, 2020

Prenylation is an important modification on peripheral membrane binding proteins. Insect cells can be manipulated to produce farnesylated and carboxymethylated KRAS4b in quantities that enable biophysical measurements of protein-protein and protein-lipid interactions

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