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Volledig verwerkte recombinant KRAS4b
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Volledig verwerkte recombinant KRAS4b: isoleren en karakteriseren van het Farnesylated en gemethyleerd eiwit

Article DOI: 10.3791/60703-v • 07:08 min • January 16th, 2020
January 16th, 2020 •
1, 1, 1, 1, 1, 1, 1
1NCI RAS Initiative, Cancer Research Technology Program, Frederick National Laboratory for Cancer Research

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Prenylatie is een belangrijke modificatie op perifere membraan bindende eiwitten. Insecten cellen kunnen worden gemanipuleerd om gefarnesylen carboxymethylated KRAS4b te produceren in hoeveelheden die biofysische metingen van eiwit-eiwit-en proteïne-lipide-interacties mogelijk maken

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Biochemie uitgave 155 insect cel eiwitproductie carboxymethylatie farnesylation intact spectrometrie inheemse massaspectrometrie oppervlakte Plasmon resonantie liposomen
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