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Journal / Biochemistry / Tam İşlenmiş Rekombinant KRAS4b: Farnesile ve…
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Biochemistry

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JoVE Journal Biochemistry
Tam İşlenmiş Rekombinant KRAS4b
A Guide to Production, Crystallization, and Structure Determination of Human IKK1/α
Journal (Biochemistry)
A Guide to Production, Crystallization, and Structure Determination of Human IKK1/α
Preparation of Nucleosome Core Particles Complexed with DNA Repair Factors for Cryo-Electron Microscopy Structural Determination
Journal (Biochemistry)
Preparation of Nucleosome Core Particles Complexed with DNA Repair Factors for Cryo-Electron Microscopy Structural Determination
 
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Tam İşlenmiş Rekombinant KRAS4b: Farnesile ve Metillenmiş Proteini Yalıtma ve Karakterize Etme

Article DOI: 10.3791/60703-v • 07:08 min • January 16th, 2020
January 16th, 2020 •
1, 1, 1, 1, 1, 1, 1
1NCI RAS Initiative, Cancer Research Technology Program, Frederick National Laboratory for Cancer Research

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Prenilasyon periferik membran bağlayıcı proteinler üzerinde önemli bir değişikliktir. Böcek hücreleri, protein-protein ve protein-lipid etkileşimlerinin biyofiziksel ölçümlerini sağlayan miktarlarda farnesile tedavüle ve karboksimetilatlanmış KRAS4b üretmek için manipüle edilebilir.

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Biyokimya Sayı 155 böcek hücre proteini üretimi karboksimetilasyon farnesilasyon sağlam spektrometre yerli kütle spektrometresi yüzey plazmon rezonansı lipozomlar
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