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Journal / Biochemistry / Fullt behandlet rekombinant KRAS4b: isolere og…
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JoVE Journal Biochemistry
Fullt behandlet rekombinant KRAS4b
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Fullt behandlet rekombinant KRAS4b: isolere og karakteriserer Farnesylated og denaturert protein

Article DOI: 10.3791/60703-v • 07:08 min • January 16th, 2020
January 16th, 2020 •
1, 1, 1, 1, 1, 1, 1
1NCI RAS Initiative, Cancer Research Technology Program, Frederick National Laboratory for Cancer Research

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Prenylation er en viktig modifikasjon på perifere membran bindende proteiner. Insekt celler kan manipuleres til å produsere farnesylated og carboxymethylated KRAS4b i mengder som muliggjør Biofysiske målinger av protein-protein og protein-lipid interaksjoner

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Biokjemi utstede 155 insekt celle protein produksjon carboxymethylation farnesylation intakt massespektrometri Native Mass massespektrometri overflate Plasmon resonans liposomer
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