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JoVE Journal
Immunology and Infection

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基于 TurboID 的接近标签,用于蛋白质-蛋白质相互作用网络的植物中识别
 
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基于 TurboID 的接近标签,用于蛋白质-蛋白质相互作用网络的植物中识别

Article doi: 10.3791/60728
May 17th, 2020

Summary May 17th, 2020

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这里描述的是一种接近标记方法,用于识别尼科蒂亚娜本查米亚叶组织中NLR免疫受体的TIR域的相互作用伙伴。还提供了一个详细的协议,用于识别其他感兴趣的蛋白质之间的相互作用使用这种技术在尼科蒂亚纳和其他植物物种。

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