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VIGS介导的正向遗传学筛选参与非寄主抗性基因鉴定
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Immunology and Infection
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JoVE Journal Immunology and Infection
VIGS-Mediated Forward Genetics Screening for Identification of Genes Involved in Nonhost Resistance
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VIGS介导的正向遗传学筛选参与非寄主抗性基因鉴定

DOI:
10.3791/51033-v

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08:17 min

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August 23, 2013

•

, ,
1Plant Biology Division,The Samuel Roberts Noble Foundation

Chapters

  • 00:05Title
  • 01:32Plant Growth and Target Gene Silencing
  • 03:22Preparation of Nonhost Pathogen Cultures of Plant Inoculation
  • 04:26Observation of in planta Growth of Bacterial Pathogens
  • 05:13Sequencing the Insert and Identification of Target Gene
  • 05:50Results: Gene-silenced N. benthamiana Plants Compromised for Nonhost Resistance
  • 07:22Conclusion

Summary

Automatic Translation

Automatic Translation

病毒诱导的基因沉默是一个有用的工具，用于识别植物非寄主抗性基因参与。我们演示了如何使用中表达GFPuv的识别基因沉默植株易受非寄主病原体的细菌病原体。这种做法是方便，快捷，有利于大规模筛选和类似协议可以应用到其他各种植物与微生物相互作用研究。

Tags

VIGSVirus-induced Gene SilencingForward Genetics ScreeningNonhost ResistanceNicotiana BenthamianaPseudomonas SyringaeXanthomonas CampestrisGFPuvPlant Defense Genes

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